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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in several tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to guarantee the absolute best results are...
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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope about the concentrate on protein. By evaluating the staining in the blocked antibody compared to the antibody on your own, one can see read more which staining is precise.
Concentrate on Data Cyclin E belongs for the extremely conserved cyclin relatives, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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